Real-Time Kinetic Biomarkers by IncuCyte S3
Real-Time Kinetic Biomarkers by IncuCyte S3
Static endpoint assays often miss the critical temporal dynamics of cellular behavior, limiting the depth of insight into therapeutic mechanisms. We utilize the Sartorius IncuCyte S3® Live-Cell Analysis System to automatically and continually collect and analyze images throughout the course of an experiment. Because the system operates entirely within the stable environment of a tissue culture incubator, it provides real-time, quantitative analysis of live cells without ever displacing them or perturbing their physiological surroundings.
Our bespoke live-cell assay portfolio supports robust decision-making across advanced biotherapeutic development:
Immune Cell Killing Assays:
Evaluate the efficacy, exhaustion, and kinetics of immune cell targeting. This is critical for assessing T-cell therapies and targeted immunomodulators.
Targeted Cytotoxicity:
Continuously track specific immune responses and target-cell clearance in real-time to fully characterize complex immune-tumor cell interactions.
Real-Time Proliferation & Viability:
Continuously monitor cell growth, confluence, and accurate cell counts without disturbing the culture environment.
Apoptosis & Cytotoxicity Tracking:
Utilize optimized, non-perturbing fluorescent reagents (such as Caspase-3/7 markers) to dynamically quantify apoptotic pathways and drug-induced toxicity over days or weeks.
Cell Migration & Invasion:
Analyze metastatic potential and localized tissue repair mechanisms through automated scratch-wound models.
3D-Spheroid Analysis:
Evaluate therapeutic penetration, viability, and growth kinetics within highly physiological 3D spheroid models.
Reporter Gene Expression & Viral Studies
Track transduction efficiency and dynamic gene expression. This offers critical kinetic readouts for assessing novel non-viral gene delivery systems and custom cell and gene therapies.
